Storage of biological material
In scientific research, biological samples -such as blood, tissue, urine, saliva, cell cultures, microorganisms, DNA, or seeds- are essential for advancing knowledge and finding solutions to health, environmental and biodiversity-related problems. To be useful, these samples must be stored correctly in accordance with standards that guarantee their quality, ensure the safety of those handling them and guarantee compliance with current legislation.
At the UAB, biological samples are stored under controlled conditions to ensure they remain in good condition for future studies. This involves storing them in suitable locations with specialised equipment and controlling temperature and other environmental factors. Detailed records are kept for each sample. The entire process is carried out responsibly, in accordance with ethical and legal criteria, to protect both data and people.
What requirements must be met in order to store biological samples at the UAB?
In order to guarantee the quality, traceability and safety of these samples, the following must be done:
- Classify and label samples correctly.
- Record them in a database with detailed information.
- Restrict access to authorised personnel only.
- Comply with biosafety, data protection and ethics regulations.
What preservation methods are used for biological samples?
The most common methods are:
- Refrigeration (2–8 °C): for short-term preservation.
- Freezing (-20°C to -75°C): for long-term preservation.
- Cryopreservation (<-150°C): for living cells.
- Lyophilization, fixation and dehydration: depending on the type of sample and its purpose.
What safety measures should be applied when receiving samples?
- Verify the integrity and identification of the samples.
- Assign a unique, non-linkable code.
- Use appropriate PPE (gloves, lab coat, goggles).
- Disinfect surfaces and containers.
- Apply quarantine to new biological agents.
- Comply with general biosafety regulations.
What are the most common mistakes when storing biological samples?
Common mistakes include:
- Alteration of environmental conditions due to temperature, humidity, or light fluctuations.Solution: Regularly monitor conditions and use appropriate preservation equipment.
- Expiration of the shelf life.Solution: Record entry and expiration dates and dispose of expired samples following biosafety regulations.
- Loss or theft of samples.Solution: Implement security systems, entry/exit logs and restricted access control.
- Incorrect identification.Solution: Assign unique codes, use indelible labels and verify consistency with documentation.
- Accidental contamination.Solution: Apply biosafety protocols, use PPE, and disinfect surfaces and containers.