Nuclear Magnetic Resonance (SeRMN)

The Nuclear Magnetic Resonance (SeRMN) Service's main objective is to carry out and facilitate the acquisition, processing, analysis and interpretation of spectroscopic and imaging data obtained using nuclear magnetic resonance (NMR) techniques.

Services

• Recording of mono- and multidimensional multinuclear NMR spectra of molecules in solution for the structural characterisation of chemical compounds and the resolution of analytical problems. 250 MHz to 600 MHz spectrometers.
• Structural and dynamic analysis of biomolecules (peptides, proteins, nucleic acids, etc.) in solution. Ligand-protein interaction studies.
• Analysis of complex mixtures. Component identification. Impurity detection and characterisation. Quantitative analysis.
• Metabolomic studies in the fields of food science and technology, biomedicine, and the pharmaceutical industry.
• 400 MHz solid-state multinuclear NMR analysis (CP/MAS).
• Analysis of semi-solid/semi-liquid samples by high-resolution magic-angle spinning (HR-MAS) techniques (1H, 13C and 31P) at 400 MHz.
• Characterisation of mixtures by the combined and automated use of liquid chromatography, nuclear magnetic resonance, and cryoprobe mass spectrometry techniques (1H, 13C and 15N HPLC-NMR/MS) at 500 MHz.
• Non-invasive analysis of small animals, foodstuffs and plants using magnetic resonance imaging (MRI) and localised 1H, 13C and 31P spectroscopy at 7 Tesla.
• Application of hyperpolarisation techniques using dynamic nuclear polarisation (DNP) to increase sensitivity in 13C nuclear magnetic resonance experiments in vitro at 600 MHz and in vivo at 7 Tesla.