Flow Induced Dispersion Analysis

In response to the needs of users of the Genomics and Biomolecular Spectroscopy Service (SGiEB), it has acquired a FIDA system. The acquisition was made possible by the infrastructure grant from the Ministry of Science, Innovation and Universities, reference EQC2024-008815-P.

What is FIDA?

FIDA stands for Flow Induced Dispersion Analysis. This new technology allows the conformational changes of an analyte in solution to be analysed by measuring the dispersion of intrinsic (proteins) or extrinsic (covalent fluorescent labelling) fluorescent light emitted by the compound. This allows the evaluation of numerous aspects that can affect the conformational behaviour of the analyte, such as the interaction of a cofactor or ligand, changes due to variations in ionic strength, pH or temperature... With this data, FIDA allows us to obtain information on the hydrodynamic radius of the molecules and to perform calculations on the association or dissociation constants between molecules, concentration measurements, sample integrity... and always in solution. The acquired instrument features a Peltier temperature control system, which also allows for thermal stability analysis. The analysis time is a few minutes and the required sample quantity is very low (in the nM or pM range) due to the instrument's high sensitivity.

For more information, you can consult their website where you will find detailed information and videos.